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运用STR-PCR、变性聚丙烯酰胺凝胶电泳结合荧光DNA自动检测技术对广州汉族人群6个STR位点(vWA31A、TH01、F13A01、FES、TPOX和CSF1PO)等位基因频率和基因型频率进行了调查,并与其它人群的等位基因频率进行了对比。所有位点的结果均符合Hardy-Weinberg平衡,且各位点等位基因间无相关性。本方法可靠、灵敏,所得的等位基因频率等数据可为汉族人群法医个人识别、亲子鉴定及遗传学研究提供基础数据。
The frequencies of alleles and genotypes in 6 STR loci (vWA31A, TH01, F13A01, FES, TPOX and CSF1PO) in Guangzhou Han population were analyzed by STR-PCR, denaturing polyacrylamide gel electrophoresis combined with automated fluorescent DNA detection Surveys were compared with allele frequencies in other populations. Results at all loci were in accordance with the Hardy-Weinberg equilibrium with no association between alleles at each locus. The method is reliable and sensitive, and the obtained allele frequency and other data can provide basic data for the foreigners forensic personal identification, paternity testing and genetics research.