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目的观察日本血吸虫未成熟虫卵发育过程中形态学变化,深入了解抗卵胚免疫作用的效应机制。方法应用透射电镜及免疫电镜方法,研究日本血吸虫卵及其肉芽肿反应超微结构,并对成熟虫卵及未成熟虫卵进行透射电镜和免疫电镜比较观察。结果进一步证实日本血吸虫卵壳表面布满网络状纤丝和微棘状突起。成熟虫卵纤毛微管呈现典型的9+2结构。肉芽肿细胞形态超微结构显示嗜酸性粒细胞、巨噬细胞、浆细胞、淋巴细胞和纤维母细胞功能活跃。结论提示日本血吸虫卵肉芽肿的形成是细胞免疫与体液免疫联合参与的结果。经免疫电镜证明,抗未成熟卵抗体分子和嗜酸性粒细胞特殊颗粒在抗卵胚发育免疫中具有潜在的意义。
Objective To observe the morphological changes of Schistosoma japonicum immature egg during development and to understand the effect mechanism of anti-egg immunity. Methods The transmission electron microscopy and immunoelectron microscopy were used to study the ultrastructure of Schistosoma japonicum eggs and granuloma reaction. The mature eggs and immature eggs were observed by transmission electron microscopy and immunoelectron microscopy. The results further confirmed that the Schistosoma japonicum eggshell surface covered with network fibrils and spiny spikes. Mature oocysts microtubules showed a typical 9 +2 structure. Granuloma cell ultrastructure showed eosinophils, macrophages, plasma cells, lymphocytes and fibroblasts functionally active. Conclusions suggest that the formation of Schistosoma japonicum egg granuloma is a result of a combination of cellular and humoral immunity. The immunoelectron microscopy proved that the anti-immature egg antibody molecule and the special granules of eosinophils have potential significance in the anti-egg developmental immunity.