论文部分内容阅读
通过对5种大孔树脂的对比研究,筛选出一种对黄姜中薯蓣皂苷吸附性能与洗脱性能最佳的树脂,并确定了树脂纯化薯蓣皂苷的最佳工艺参数。利用超声波-协同表面活性剂萃取得到皂苷粗提液,利用分光光度法测定样品中薯蓣皂苷的含量,考察上样浓度、吸附流速、洗脱剂浓度、洗脱量、洗脱流速、树脂重复使用次数等条件对吸附与洗脱结果的影响。结果表明D101大孔树脂对黄姜中薯蓣皂苷的纯化效果较好。其最佳工艺条件为上样浓度为粗提液浓度,吸附流速为1BV/h,洗脱剂用量为50mL,洗脱剂浓度为70%的乙醇,洗脱流速为3BV/h,大孔树脂重复利用次数最佳为4次,薯蓣皂苷的纯度和收率分别可达到40%和94.02%。该工艺简单可行,精制效果好,适用于工业化生产。
By comparing the five kinds of macroporous resins, a resin with the best adsorption and elution properties of diosgenin in turmeric was screened out and the optimum technological parameters of resin-purified diosgenin were determined. The saponin crude extract was obtained by ultrasonic-co-surfactant extraction. The content of diosgenin in the sample was determined by spectrophotometry. The effects of sample concentration, adsorption flow rate, eluent concentration, elution volume, elution flow rate, repeated use of resin The number of conditions such as adsorption and elution results. The results showed that the D101 macroporous resin purify diosgenin in turmeric ginger better. The optimum conditions for the sample concentration of the crude extract concentration, the adsorption flow rate of 1BV / h, the amount of eluant was 50mL, the eluent concentration of 70% ethanol, the elution flow rate of 3BV / h, macroporous resin The best reuse was 4 times. The purity and yield of diosgenin reached 40% and 94.02%, respectively. The process is simple and feasible, refined effect, suitable for industrial production.