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目的研究人食管鳞状细胞癌与癌旁正常组织的基因表达谱,通过对比基因表达的差异,寻找与人食管鳞癌发展相关的基因。方法提取人食管鳞癌与正常食管鳞状上皮的mRNA,逆转录成cDNA,并用Cy3-dUTP标记正常食管组织,用Cy5-dUTP标记食管鳞癌组织,制成探针,与基因芯片进行杂交,经扫描仪扫描后,对获取的信号数据进行软件分析、对比,筛选出差异表达的基因。结果在4例新鲜标本的芯片杂交检测中,共发现4329条基因发生差异表达,其中2293条上调,2036条下调,将这些基因按GO(gene ontology)分子功能(function term)进行分类,发现与细胞分化、成熟,分子定位、连接,信号传导,酶活性调节,以及基因转录、翻译调节有关的基因最多。结论①食管鳞癌的发生发展与多条基因的异常表达有关。②应用基因芯片技术可以对食管鳞癌的基因表达谱进行分析,以筛选食管鳞癌相关基因。
Objective To study the gene expression profile of human esophageal squamous cell carcinoma and adjacent normal tissues, and to find the genes related to the development of human esophageal squamous cell carcinoma by comparing the differences in gene expression. Methods The mRNA of human esophageal squamous cell carcinoma and normal esophageal squamous epithelium was extracted and reverse transcribed into cDNA. The normal esophageal tissue was labeled with Cy3-dUTP, and esophageal squamous cell carcinoma was labeled with Cy5-dUTP to make a probe to hybridize with the gene chip. After scanning by the scanner, the obtained signal data were analyzed by software, and the differentially expressed genes were screened out. Results A total of 4329 genes were found to be differentially expressed in 4 samples of fresh samples. Among them, 2293 were up-regulated and 2036 were down-regulated. These genes were classified according to GO (gene ontology) molecular function, Cell differentiation, maturation, molecular location, connectivity, signaling, regulation of enzyme activity, and gene transcription, translation regulation-related genes up. Conclusion ① The occurrence and development of esophageal squamous cell carcinoma are related to the abnormal expression of multiple genes. ② gene chip technology can be used to analyze the gene expression profile of esophageal squamous cell carcinoma to screen esophageal squamous cell carcinoma related genes.