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以活力、大小一致的菖蒲小植株为试材,以不添加铬的营养液为对照(CK),研究了5、10、20、40、60mg/L铬(Ⅵ)胁迫对菖蒲(Acorus calamus)根系活力(TTC)、叶绿素含量、维生素C含量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性等生理生化特性的影响,为其作为水体环境修复材料的可行性提供科学参考。结果表明:菖蒲在低、中浓度(5~20mg/L)铬(Ⅵ)胁迫时叶绿素含量有所增加,而在高浓度(40、60mg/L)时显著下降;随铬(Ⅵ)胁迫浓度的增加,可溶性蛋白质含量总体上显著上升,可溶性糖含量总体上呈下降趋势,脯氨酸含量先升后降,而维生素C含量在低浓度时降幅较小,在高浓度时降幅较大。随着铬(Ⅵ)处理浓度的增加,菖蒲根系活力、还原型谷光甘肽(GSH)含量、MDA含量均为先增后降,而超氧阴离子自由基含量先降后增;菖蒲SOD活性与苯丙氨酸解氨酶(PAL)活性均随着铬(Ⅵ)浓度的增加呈先增后降趋势,而CAT活性和POD活性均显著增加;综上,菖蒲能适应一定浓度的铬胁迫并做出相应的调节,从而表现出较强的适应力和耐污力,可以作为人工湿地处理含铬废水的物种之一。
The effects of 5,10,20,40 and 60 mg / L Chromium (Ⅵ) stress on the growth of Acorus calamus were studied with irrigating solution without CK as CK, Rooting activity (TTC), chlorophyll content, vitamin C content, malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, peroxidase (POD) activity and catalase Biochemical characteristics of its impact on the environment as a water remediation materials to provide a scientific reference for the feasibility. The results showed that: Acorus calamus increased chlorophyll content in low and medium concentration (5 ~ 20mg / L) Cr (Ⅵ) stress, but significantly decreased in high concentration (40 and 60mg / L) The content of soluble protein increased as a whole. The content of soluble sugar decreased first and then decreased, while the content of vitamin C decreased slightly at low concentration and declined more at high concentration. With the increase of Cr (Ⅵ) concentration, root activity, GSH content and MDA content of A. cantonensis increased first and then decreased, while the content of superoxide anion radical decreased first and then increased; Phenylalanine ammonia lyase (PAL) activity increased first and then decreased with increasing Cr (Ⅵ) concentration, and CAT activity and POD activity increased significantly. In conclusion, iris can adapt to a certain concentration of chromium stress Make the corresponding adjustment, so as to show strong adaptability and fouling resistance, and can be used as one of the species for treating chromium-containing wastewater in constructed wetlands.