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目的探讨云南青阳参居群内、居群间和种子间的遗传多样性。方法利用AFLP分子标记技术检测云南不同生态环境中青阳参居群间、居群内及不同植株间和同一植株不同双生蓇葖果的不同种子间遗传多样性。结果居群内遗传多样性比居群间遗传多样性丰富,居群间有效基因等位数(Ne)、Nei’s基因多样性(H)、Shannon’s指数(I)分别为1.172、0.105、0.161,居群内最高为1.217、0.121、0.177;种子之间遗传多样性H值为0.095,I值为0.145)高于双生蓇葖果间(H值为0.121,I值为0.182)。结论云南青阳参遗传多样性较低,需要进行保护;在云南3个生态环境中,楚雄的青阳参遗传多样性较其他地区丰富,AFLP图谱中也出现与该生态环境相一致的特异条带。
Objective To explore the genetic diversity among populations of Gynura yunnanensis, populations and seeds in Yunnan. Methods AFLP molecular markers were used to detect the genetic diversity among different populations of different populations in different ecological environments in Yunnan Province, among populations and between different plants and different twins of the same plant. Results The genetic diversity within the population was more abundant than that among the populations. The number of available genes (Ne), Nei’s gene diversity (H) and Shannon’s index (I) were 1.172, 0.105 and 0.161, respectively The highest within the group was 1.217,0.121,0.177; the genetic diversity between seeds was 0.095, the I value was 0.145) was higher than that between twins (H value 0.121, I value 0.182). Conclusion The genetic diversity of P. yunnanensis is low and needs to be protected. Among the three eco-environments in Yunnan, the genetic diversity of C. chinensis is more abundant than that of other regions, and AFLP maps also show the same specificities band.