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目的:分析三氧化二砷(ATO)对血小板聚集功能的影响。方法:用不同浓度的ATO(0、0.25、0.5、1、1.5、2、2.5、5和10μmol/L)孵育富血小板血浆(PRP)0、3、15、30、45和60 min,在2 mg/L胶原或2μmol/L腺苷二磷酸(ADP)刺激下,检测血小板聚集功能。结果:在刺激剂为2 mg/L胶原时,ATO(≥5μmol/L)孵育PRP 60 min,可显著抑制血小板聚集,在相应的时间梯度实验中,ATO浓度从5μmol/L上升到10μmol/L,抑制血小板聚集所需要的孵育时间从45 min下降到30 min,ATO对胶原诱导的血小板聚集具有浓度(r=-0.902,P=0.001)和时间(r=-0.964,P=0.002;r=-0.910,P=0.032)依赖性;在刺激剂为2μmol/L ADP时,ATO(≥2μmol/L)孵育PRP 60 min,可显著抑制血小板聚集,在相应的时间梯度实验中,ATO浓度从5μmol/L上升到10μmol/L,抑制血小板聚集所需要的孵育时间从45 min下降到15 min,ATO对ADP诱导的血小板聚集亦具有浓度(r=-0.815,P=0.007)和时间(r=-0.921,P=0.009;r=-0.963,P=0.009)依赖性。结论:低浓度的ATO对血小板聚集功能没有明显的作用,但是高浓度ATO(≥2μmol/L)可抑制血小板的聚集功能,并且该抑制作用具有浓度和时间依赖性。
Objective: To analyze the effect of arsenic trioxide (ATO) on platelet aggregation. Methods: Platelet-rich plasma (PRP) was incubated with different concentrations of ATO (0,0.25,0.5,1,1.5,2,2.5,5 and 10 μmol / L) for 0, 3, 15, 30, 45 and 60 min, Platelet aggregation function was detected by stimulating with 1 mg / L collagen or 2 μmol / L adenosine diphosphate (ADP). Results: At a stimulant of 2 mg / L collagen, ATO (≥5 μmol / L) incubated with PRP for 60 min significantly inhibited platelet aggregation. In the corresponding time-gradient experiments, the concentration of ATO increased from 5 μmol / L to 10 μmol / L (R = -0.902, P = 0.001) and time (r = -0.964, P = 0.002; r = -0.902, P = 0.002). The time required for the inhibition of platelet aggregation decreased from 45 min to 30 min. The concentration of ATO on collagen-induced platelet aggregation -0.910, P = 0.032). When stimulated with 2μmol / L ADP, ATO (≥2μmol / L) incubated PRP for 60min significantly inhibited platelet aggregation. In the corresponding time-gradient experiments, the concentration of ATO increased from 5μmol / L to 10μmol / L, the incubation time needed to inhibit platelet aggregation decreased from 45 min to 15 min, and ATO also had a concentration of ADP - induced platelet aggregation (r = -0.815, P = 0.007) 0.921, P = 0.009; r = -0.963, P = 0.009). CONCLUSION: Low concentration of ATO has no obvious effect on platelet aggregation, but high concentration of ATO (≥2μmol / L) can inhibit platelet aggregation, and the inhibition is concentration-dependent and time-dependent.