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建立了基于表面活性剂浊点萃取-反相高效液相色谱法同时分离测定小儿泻速停颗粒中没食子酸、儿茶素、表儿茶素的方法。采用phenomenex C18色谱柱,流动相为乙腈-0.2%冰乙酸水溶液,采用梯度洗脱;流速0.8 mL/min;检测波长280 nm。没食子酸、儿茶素和表儿茶素分别在0.0104～223μg/mL,(r=0.9999);0.0152～108μg/mL,(r=0.9999);0.0184～132μg/mL,(r=0.9999)范围内线性关系良好,定性检测限(S/N=3)依次为:0.087 ng/mL,0.43 ng/mL,0.42 ng/mL。加入50 g/L NaCl来进行浊点富集,可提高萃取回收率。平均回收率为91.85%～98.48%,本方法可用于该制剂的质量控制。
A method for the simultaneous determination of gallic acid, catechin and epicatechin in children by using cloud point extraction and reversed-phase high performance liquid chromatography (RP-HPLC) was established. Phenomenex C18 column was used. The mobile phase consisted of acetonitrile-0.2% glacial acetic acid with gradient elution. The flow rate was 0.8 mL / min. The detection wavelength was 280 nm. (R = 0.9999), 0.0152 ~ 108μg / mL, (r = 0.9999), 0.0184 ~ 132μg / mL, (r = 0.9999) in the range of 0.0104 ~ 223μg / mL, The linearity was good. The qualitative detection limits (S / N = 3) were 0.087 ng / mL, 0.43 ng / mL and 0.42 ng / mL, respectively. Add 50 g / L NaCl for cloud point enrichment, can improve the extraction recovery. The average recoveries ranged from 91.85% to 98.48%. This method can be used for the quality control of this preparation.