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目的分析玻璃化冷冻保存技术在人类卵裂期胚胎的应用价值,观察临床效果。方法回顾性分析2011年1月~2012年12月期间在我院生殖中心行玻璃化冷冻解冻患者共272个周期的妊娠情况。结果玻璃化冷冻解冻共657枚胚胎,全部卵裂球存活612枚(93.15%),存活率98.33%(646/657);共冷冻解冻272个周期,均有存活胚胎可移植,妊娠率37.13%(101/272),种植率19.97%(129/646)。结论玻璃化冷冻保存技术在人类卵裂期胚胎中可取得较好的临床效果,与新鲜胚胎移植妊娠结局相当。
Objective To analyze the value of vitrification cryopreservation in human cleavage stage embryos and to observe the clinical effect. Methods Retrospective analysis of pregnancy between January 2011 and December 2012 in 272 cycles of vitrification and thawing in our reproductive center. RESULTS: A total of 657 embryos were frozen and thawed with 612 surviving blastocysts (93.15%) and surviving rate of 98.33% (646/657). All of the surviving embryos were transplanted in 272 cycles of freezing and thawing. The pregnancy rate was 37.13% (101/272), the planting rate of 19.97% (129/646). Conclusion Vitrified cryopreservation technique has good clinical effect in human cleavage stage embryo, which is comparable with the pregnancy outcome of fresh embryo transfer.