续上报,对黄柏果实中的生物碱成分进行了筛选,并与原植物及愈伤组织的生成成分进行了比较。 将黄柏果实(fw 2kg)粉碎,以甲醇提取,其浓缩提取物以氯仿、含水丁醇依次提取,其中的氯仿可溶部分上SiO_2及LH-20柱反复纯化,分离出小檗碱以外的2种生物碱(1)与(2)。经各种仪器数据分析以及与标准品比较,鉴定(1)为吴茱萸次碱。(2)的MS检测数据为m/z 303(M~-)、285(M~+-18),IR检测认为存在OH。~1H-NMR(CDCl_3,δ,ppm)6.88处 In the report, the alkaloids in Phellodendron chinense were screened and compared with the original plant and calli. The Phellodendron chinense fruit (fw 2kg) was crushed and extracted with methanol. The concentrated extract was extracted sequentially with chloroform and water butanol. The chloroform soluble part was repeatedly purified on SiO 2 and LH-20 columns to separate the berberine. Alkaloids (1) and (2). Through various instrumental data analysis and comparison with the standard product, identification (1) is rutacarpine. The MS detection data of (2) is m/z 303 (M~-), 285 (M~+-18), and IR detection indicates the presence of OH. ~1H-NMR (CDCl_3, δ, ppm) 6.88