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目的:探讨丙泊酚预处理对大鼠布比卡因中枢神经系统及心脏毒性的影响。方法:雄性SD大鼠20只随机分为对照组和丙泊酚预处理组,每组各10只,分别于静脉泵注布比卡因前30min,ip生理盐水10ml/kg或10g/L丙泊酚100mg/kg(10ml/kg)。以肢体Ⅱ导联监测ECG,暴露大鼠股动脉,置入24G套管针监测动脉血压和备抽取血样,同时暴露大鼠股静脉,置入24G套管针泵入布比卡因。记录各基础参数后,开始用微量注射泵泵入5g/L布比卡因2mg/(kg.min),记录动物发生抽搐、ECG出现心律失常及心搏停止的时间,计算布比卡因在相应时间点的累积剂量。结果:泵注布比卡因后,丙泊酚预处理组大鼠发生抽搐、心律失常及心搏停止时,布比卡因的累积剂量分别为:(8.9±2.1)mg/kg,(10.5±2.8)mg/kg及(28.0±4.9)mg/kg,均明显大于对照组大鼠的剂量(7.2±2.1)mg/kg,(8.5±2.5)mg/kg及(22.4±3.4)mg/kg(P<0.05)。结论:丙泊酚预处理可提高大鼠对布比卡因中枢神经及心脏毒性的耐受。
Objective: To investigate the effects of propofol preconditioning on central nervous system and cardiotoxicity of bupivacaine in rats. Methods: Twenty male Sprague-Dawley rats were randomly divided into control group and propofol preconditioning group, with 10 rats in each group. The rats were anesthetized with intravenous infusion of bupivacaine for 30min, ip saline 10ml / kg or 10g / L Propofol 100 mg / kg (10 ml / kg). Electrocardiography (ECG) was performed on the limbs II. The femoral arteries of the rats were exposed. A 24G trocar was placed to monitor arterial blood pressure and blood samples were prepared for extraction. The femoral vein was also exposed and a 24G trocar was inserted into bupivacaine. After recording the basic parameters, begin to inject 5g / L bupivacaine 2mg / (kg.min) with the micro-injection pump, and record the twitch of animals, the arrhythmia of ECG and the time of cardiac arrest, calculate the time of bupivacaine in The cumulative dose at the appropriate time. RESULTS: After bupivacaine infusion, the cumulative doses of bupivacaine in the propofol preconditioning group were (8.9 ± 2.1) mg / kg and (10.5 ± 2.8 mg / kg and 28.0 ± 4.9 mg / kg were significantly higher than those of the control group (7.2 ± 2.1 mg / kg, (8.5 ± 2.5) mg / kg and (22.4 ± 3.4) mg / kg (P <0.05). Conclusion: Propofol preconditioning can improve the rat’s tolerance to central nervous and cardiac toxicity of bupivacaine.