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目的 研究灰旱獭感染乙型肝炎病毒 (HBV)后的血清学反应。方法 实验前普查 2 8只实验灰旱獭对乙肝病毒标志物 (HBVM)的血清学变化 ,选阴性健康旱獭用含HBVDNA的人血清进行皮下、肌内和腹腔多点注射 (4 96× 10 6cop ml× 3ml) ,接种后 1～ 8周 (每日 )和 2～ 12个月 (每 1～ 2个月 )观察行为变化 ,检测HBVM ,HBVDNA和ALT AST。结果 攻毒前抗 HBc+率为 7 1% (2 2 8) ,攻毒后抗 HBc+率为 31 8% ,HBsAg+率为 4 3% ,HBVDNA+率为 16 6 % ,ALT一周后开始升高 ,2～ 3个月达高峰 (高于正常 2～ 9倍 )并持续 6个月 ,AST低平曲线高峰迟发一个月。全程死亡 7只 (2 5 % ) ,攻毒后死亡 1只 (1 7)。结论 攻毒后HBV和ALT AST的变化提示HBV感染后能产生肝细胞炎症反应
Objective To study the serological response of gray mute to hepatitis B virus (HBV) infection. Methods Serum changes of hepatitis B virus markers (HBVM) were detected in 28 experimental gray crayfish (Oryza sativa L.) before and after the experiment. Negative healthy marmots were injected subcutaneously, intramuscularly and intraperitoneally with human serum containing HBVDNA (4 96 × 10 6 cop ml × 3ml), 1 ~ 8 weeks after inoculation (daily) and 2 ~ 12 months (every 1 ~ 2 months) were observed behavior changes, detection of HBVM, HBVDNA and ALT AST. Results The anti-HBc positive rate was 71% (2 2 8) before challenge, the anti-HBc + rate was 31 8%, the HBsAg + rate was 43% and the HBVDNA + rate was 16 6% ~ 3 months to reach the peak (2 to 9 times higher than normal) and lasted 6 months, AST low curve peak late one month. Seven died (25%) and one died after challenge (17). Conclusion The changes of HBV and ALT AST after challenge suggest that the HBV infection can produce hepatocyte inflammatory reaction