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目的观察心肺复苏(CPR)效果,并探讨窒息时间及CPR时间对大鼠自主循环恢复(ROSC)率和6h生存率的影响。方法采用呼气末夹闭气管窒息法,建立大鼠心脏停搏(A)模型。30只SpragueDawley(SD)大鼠随机分2组,窒息7min组(n=15),窒息9min组(n=15)。观察大鼠窒息及心肺复苏期间平均动脉压(MAP)、心电图(ECG)、心率(HR)和动脉血pH值的变化,比较各组的ROSC率和6h生存率。结果窒息7min组ROSC率和6h存活率与窒息9min组比较,差异无显著性(P>0.05);CPR时间<5min者6h生存率显著高于CPR时间>5min者(P<0.05);存活时间大于6h者CPR时间与存活时间小于6h者CPR时间比较有显著性差别(P<0.05)。结论该复苏模型制作简单,重复性好,可观察多个指标,能满足心肺复苏实验研究的需要;窒息时间选择应控制在7～9min以内;CPR时间是预测CPR成功率的重要指标。
Objective To observe the effects of cardiopulmonary resuscitation (CPR) and to investigate the effects of asphyxia time and CPR time on spontaneous circulation recovery (ROSC) and 6h survival rate in rats. Methods Cardiac arrest (A) model was established by end-tidal tracheal asphyxia. Thirty Sprague Dawley (SD) rats were randomly divided into 2 groups. The asphyxiated 7-min group (n = 15) and the asphyxia 9-min group (n = 15). The changes of mean arterial pressure (MAP), electrocardiogram (ECG), heart rate (HR) and arterial blood pH during asphyxia and cardiopulmonary resuscitation were observed. The ROSC rate and 6-h survival rate were compared between groups. Results There was no significant difference in ROSC rate and 6h survival rate between asphyxia and asphyxia 9min group (P> 0.05). The survival rate of 6h CPR time <5min was significantly higher than that of CPR time> 5min (P <0.05) CPR time of more than 6h and survival time of less than 6h CPR time was significantly different (P <0.05). Conclusion The recovery model is simple, reproducible, and can observe a number of indicators to meet the needs of CPR experimental study; the choice of asphyxia time should be controlled within 7 ~ 9min; CPR time is an important index to predict the success rate of CPR.