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目的探讨Cyclin G在胃癌组织中对p53-鼠双微基因2(Mdm2)负反馈调节循环中的作用及可能的相互关系。方法应用免疫组织化学SP法检测48例胃癌及相应癌旁组织Cyclin G、Mdm2和p53的表达情况。结果Cyclin G高表达于胃癌细胞核。Cyclin G,Mdm2和p53阳性率分别为64．6%、47．9%、29．1%,显著高于相应的癌旁组织(P＜0．05),Cyclin G与Mdm2呈高相关性(Kappa=0．506),与p53呈低相关性(Kappa=-0．364),并且和胃癌组织学分类和Borrmann分型无关(P＞0．05)。结论胃癌组织中Cyclin G、Mdm2和p53的异常表达可能与胃癌的发生与发展有关。Cyclin G通过Mdm2调节p53及其通路,可以作为胃癌基因治疗的靶点。
Objective To investigate the role of Cyclin G in the negative feedback regulation of p53-murine double microgene 2 (Mdm2) in gastric cancer and its possible relationship. Methods The expression of Cyclin G, Mdm2 and p53 in 48 cases of gastric cancer and corresponding paracancerous tissues was detected by immunohistochemical SP method. Results Cyclin G was highly expressed in gastric cancer cells. The positive rates of Cyclin G, Mdm2 and p53 were 64.6%, 47.9% and 29.1%, respectively, which were significantly higher than those in corresponding paracancerous tissues (P <0.05). Cyclin G and Mdm2 were highly correlated Kappa = 0.506), which was negatively correlated with p53 (Kappa = -0.364), and had no correlation with histological classification and Borrmann classification (P> 0.05). Conclusion Abnormal expression of Cyclin G, Mdm2 and p53 in gastric cancer may be related to the occurrence and development of gastric cancer. Cyclin G regulates p53 and its pathway through Mdm2, which can be used as a target for gastric cancer gene therapy.