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干细胞因子(SCF)参与机体生长发育,调控多种细胞的生长,对造血干细胞、前B细胞、红系,粒系及巨核系细胞都有促分化作用,本试验主要探讨SCF对脐血NK前体细胞的促分化作用.本实验利用SCF,IL-2和SCF+IL-2培养脐血单个核细胞21d,动态计数单个核细胞总数,根据比例计算NK含量;并在第1,3,5,9,14和21天利用FACS检测细胞表型CD56/CD16和CD3分子;同时利用ATT法检测第1,5,9,14,和21天NK细胞的杀伤活性.实验结果显示:①SCF促进人脐血NK细胞的产生:SCF,IL-2和SCF+IL-2组中脐血单个核细胞的增殖倍数为3.5,4.1和6.8倍,根据脐血中NK细胞的含量为15%～20%,推算NK细胞的增殖倍数分别为5.3～7.0,6.15～8.2和10.2～13.6倍,而阴性对照组为2.5倍.由此可见,单独SCF不仅可以增加脐血NK细胞的绝对数量和比例,并且能够提高NK细胞对IL-2的增殖反应.②
Stem cell factor (SCF) is involved in the growth and development of the body and regulates the growth of a variety of cells, promoting differentiation of hematopoietic stem cells, pre-B cells, erythroid, granulocytic and megakaryocytic cells. In this experiment, In this experiment, UCB mononuclear cells were cultured with SCF, IL-2 and SCF + IL-2 for 21 days, the total number of mononuclear cells was counted dynamically, and the NK content was calculated according to the ratio; The cytotoxic activity of NK cells on day 1, 5, 9, 14, 21 was detected by FITC assay using FACS on days 9, 14, 21 and 21. The results showed that: Cord blood NK cell production: SCF, IL-2 and SCF + IL-2 group multiplication of cord blood mononuclear cells were 3.5, 4.1 and 6.8 times, according to the cord blood NK cell content of 15% to 20% , And the multiplication of NK cells was estimated to be 5.3-7.0, 6.15-8.2 and 10.2-13.6 times, respectively, while the negative control group was 2.5 times.Therefore, SCF alone can not only increase the absolute number and proportion of NK cells in umbilical cord blood, Can increase the NK cell proliferation of IL-2 response