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[目的]探讨聚合酶链反应(PCR)在非典型临床表现的急性结膜炎病因诊断中的意义.[方法]选择非典型急性结膜炎患者44人(44眼)及正常人22人(22眼),两眼共患结膜炎者取较重一侧.提取受试者结膜囊分泌物的DNA,分为2部分,分别加入细菌引物和腺病毒引物进行2次PCR扩增,凝胶电泳后观察结果.非典型急性结膜炎组同时进行细菌培养.[结果]非典型急性结膜炎组12眼(27%)扩增出520bp条带,11眼(25%)扩增出1 004bp条带,扩增出520bp兼1 004bp条带的有3眼(7%).细菌培养法有17眼(39%)呈阳性,与PCR检测结果比较差异无统计学意义(χ2=1.77,P>0.05).正常组1眼(5%)扩增出520 bp条带,未扩增出1 004 bp条带.[结论]PCR检测对非典型急性结膜炎快速病因诊断具有临床意义.
[Objective] To explore the significance of polymerase chain reaction (PCR) in the etiological diagnosis of acute conjunctivitis atypical clinical manifestations. [Methods] Forty-four patients (44 eyes) with atypical acute conjunctivitis and 22 ), Conjunctival conjunctivitis in both eyes were taken heavier side of the extract of the conjunctival secretions of patients with DNA, divided into two parts, respectively, by adding bacterial primers and adenovirus primer 2 PCR amplification, gel electrophoresis Results The atypical acute conjunctivitis group was cultured at the same time. [Results] In the atypical acute conjunctivitis group, 520 bp bands were amplified in 12 eyes (27%) and 1 004 bp bands in 11 eyes (25%), There were 3 eyes (7%) with 520 bp and 1 004 bp bands, and 17 eyes (39%) were positive by bacterial culture method, which showed no significant difference with PCR (χ2 = 1.77, P> 0.05) 520 bp band was amplified in 1 eye (5%) in normal group, and no 1 004 bp band was amplified. [Conclusion] PCR detection has clinical significance for the rapid etiological diagnosis of atypical acute conjunctivitis.