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利用一种基于时间相关单光子计数器的双光子激发荧光寿命显微成像技术,对猪眼底视网膜色素上皮层细胞内的脂褐素和氧化黑色素颗粒的空间分布及其荧光寿命特性进行了研究,尤其对于这些色素颗粒在光致氧化环境中的荧光寿命差异进行了分析.结果表明,利用荧光寿命测量能有效区分视网膜色素上皮层细胞中的多组分荧光团,利用荧光寿命的衰减参数可分辨正常及异常的荧光现象.该方法有望发展成为一种用于眼科临床诊断及病理学研究的高灵敏度的工具,对眼底细胞随年龄增长的衰老机理的研究具有重要的意义.
The spatial distribution of lipofuscin and oxidized melanin particles in porcine fundus retinal pigment epithelial cells and their fluorescence lifetime properties were studied using a two-photon excitation fluorescence lifetime microscopy based on time-correlated single-photon counter, in particular The differences of fluorescence lifetime of these pigment particles in photoxidation environment were analyzed.The results showed that fluorescence lifetime measurement can effectively distinguish multi-component fluorophores in retinal pigment epithelium cells, and can be distinguished by using the decay parameters of fluorescence lifetime And abnormal fluorescence.This method is expected to develop into a highly sensitive tool for the clinical diagnosis and pathology of ophthalmology and is of great significance for the study on the mechanism of aging of fundus cells with age.