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目的探讨不同温度的热疗对人胃癌耐药细胞株SGC7901/DDP耐药逆转作用及可能机制。方法采用体外细胞培养技术,培养人胃癌耐药细胞株SGC7901/DDP。设计不同温度(41、42、43、44℃)热疗联合化疗处理SGC7901/DDP细胞,检测其凋亡率及细胞周期的改变,流式细胞仪、反转录聚合酶链反应(RT-PCR)检测热疗、热化疗处理前后细胞中Survivin蛋白及mRNA的表达。结果不同温度热疗后SGC7901/DDP细胞凋亡率均有所升高,43℃时最高;热疗、热化疗处理后细胞周期发生改变,G0/G1细胞逐渐增多,S期逐渐减少,G2/M期相对增多,热化疗后更为明显;热疗后尤其是热化疗后SGC7901/DDP细胞中Survivin表达明显下降(P<0.01)。结论热疗能部分逆转人胃癌细胞SGC7901/DDP的耐药效应,其机制可能与改变细胞周期及抑制Sur-vivin表达有关。
Objective To investigate the reversal effect of different temperature hyperthermia on drug resistance of human gastric cancer cell line SGC7901 / DDP and its possible mechanism. Methods Human gastric cancer cell line SGC7901 / DDP was cultured by in vitro cell culture technique. SGC7901 / DDP cells were treated with hyperthermia combined with chemotherapy at different temperatures (41,42,43,44 ℃), and the apoptosis rate and cell cycle were detected by flow cytometry and reverse transcription-polymerase chain reaction (RT-PCR) ) To detect the expression of Survivin protein and mRNA in the cells before and after hyperthermia and thermotherapy. Results The apoptotic rate of SGC7901 / DDP cells increased after hyperthermia, and reached the peak at 43 ℃. The cell cycle changed after hyperthermia and thermotherapy, the number of G0 / G1 cells gradually increased, the number of S phase decreased gradually, M phase relatively increased, more obvious after thermochemotherapy; Survivin expression in SGC7901 / DDP cells decreased significantly after hyperthermia (especially P <0.01). Conclusion Hyperthermia can partially reverse the drug resistance of human gastric cancer cell line SGC7901 / DDP. The mechanism may be related to changing the cell cycle and inhibiting the expression of Sur-vivin.