论文部分内容阅读
目的:探讨阿托伐他汀对动脉粥样硬化(atherosclerosis,AS)大鼠勃起功能(erectile function)的作用及其可能机制。方法:通过注射维生素D360万IU/kg并以高脂饲料喂养的方法建立动脉粥样硬化性勃起功能障碍(ASED)大鼠模型。用不同剂量的阿托伐他汀(5、10 mg.kg-1.d-1)干预ASED大鼠,并以西地那非作为阳性对照药。给药8周后,每组取3只大鼠测定勃起功能,分离腹主动脉HE染色观察血管形态。大鼠腹主动脉采血测定血脂水平,取大鼠阴茎海绵体组织进行组织匀浆,检测组织中丙二醛(malondialdehyde,MDA)含量,总超氧化物歧化酶(to-tal superoxide dismutase,T-SOD)及锰超氧化物歧化酶(manganese superoxide dismutase,Mn-SOD)的活力,并采用RT-PCR法检测胞外型SOD基因(SODEX)的表达。结果:阿托伐他汀显著改善ASED大鼠勃起功能和血管重构状况,增强ASED大鼠海绵体Mn-SOD活力和SODEX基因的表达,降低了MDA含量;而对血脂水平和T-SOD活力无显著影响。结论:阿托伐他汀能改善ASED大鼠勃起功能,其机制可能与改善大鼠海绵体氧化应激水平和血管重构有关。
Objective: To investigate the effect of atorvastatin on erectile function in atherosclerosis rats and its possible mechanism. Methods: A rat model of atherosclerotic erectile dysfunction (ASED) was established by injection of 1.6 million IU / kg vitamin D3 and high fat diet. ASED rats were treated with different doses of atorvastatin (5, 10 mg.kg-1.d-1) and sildenafil as positive control. After 8 weeks of administration, the erectile function was measured in 3 rats in each group. The morphology of blood vessels was observed by HE staining of the abdominal aorta. Blood samples were taken from the abdominal aorta of rats to measure the blood lipid level. The corpus cavernosum tissues of rats were homogenized and the contents of malondialdehyde (MDA), total superoxide dismutase (T- SOD) and manganese superoxide dismutase (Mn-SOD) were measured. The expression of extracellular SOD gene (SODEX) was detected by RT-PCR. Results: Atorvastatin significantly improved erectile function and vascular remodeling in ASED rats, increased Mn-SOD activity and SODEX gene expression in ASED rats, and decreased MDA content. At the same time, lipid peroxidation and T-SOD activity Significantly affected. Conclusion: Atorvastatin can improve erectile function in ASED rats, which may be related to the improvement of oxidative stress and vascular remodeling in spontaneous rats.