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目的:研究淋巴细胞活化基因-3-Ig(LAG-3-Ig)对哮喘小鼠Th1和Th2型细胞因子的比例和气道反应性的影响,为防治哮喘提供实验依据。方法:经卵蛋白(OVA)致敏的雌性BALB/c小鼠于激发前腹腔注射LAG-3-Ig50g,末次激发24h后,收集支气管肺泡灌洗液(BALF),以ELISA法测定BALF中IL-4、IL-5和IFN-γ水平。同时观察哮喘小鼠气道反应性的变化。结果:与治疗前比较,经LAG-3-Ig治疗后,哮喘小鼠BALF中IL-4和IL-5的水平显著降低(分别减少了27.9%和29.6%);而IFN-γ的水平显著增高(增加30.8%,P<0.01)。LAG-3-Ig治疗组小鼠气道螺旋条对组织胺的反应性降低,各个密度的组织胺所诱导的气管螺旋条收缩张力显著低于哮喘对照组(P<0.05)。结论:LAG-3-Ig可抑制哮喘小鼠IL-4和IL-5的分泌并能降低小鼠气道的反应性,对哮喘的治疗具有临床意义。
Objective: To study the effect of LAG-3-Ig on Th1 and Th2 type cytokines and airway responsiveness in asthmatic mice and to provide experimental evidence for the prevention and treatment of asthma. Methods: BALB / c mice were sensitized with ovariectomized (OVA) -induced BALB / c mice by intraperitoneal injection of LAG-3-Ig50g. After the last challenge, bronchoalveolar lavage fluid (BALF) was collected and IL- -4, IL-5 and IFN-γ levels. At the same time, the changes of airway responsiveness in asthmatic mice were observed. Results: Compared with those before treatment, the levels of IL-4 and IL-5 in BALF of asthmatic mice decreased significantly (by 27.9% and 29.6%, respectively) after treatment with LAG-3-Ig, while the level of IFN-γ was significantly Increase (30.8% increase, P <0.01). The responsiveness of airway spiral strips to histamine decreased in LAG-3-Ig-treated mice, and the contraction tension of tracheal spiral strips induced by histamine at various densities was significantly lower than that in asthma control group (P <0.05). Conclusion: LAG-3-Ig can inhibit the secretion of IL-4 and IL-5 in asthmatic mice and decrease the airway responsiveness in mice, which is of clinical significance for the treatment of asthma.