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目的建立固相萃取-高效液相色谱法测定土壤、麦粉和小麦植株中叶菌唑残留的方法。方法土壤、麦粉和小麦植株样品采用乙腈提取,经Silica-SPE固相萃取柱净化,Eclicpse Plus C_(18)色谱柱分离,采用高效液相色谱法进行检测,以外标法定量。结果叶菌唑在0.1~10 mg/L范围内呈良好的线性关系,标准曲线方程为Y=37.395X-1.0902,相关系数为0.9999。在加标水平为0.05~2.0 mg/kg范围内,叶菌唑在土壤、麦粉和小麦植株中的平均回收率为87.2%~100.9%,相对标准偏差为0.2%~8.7%。叶菌唑的最低检测浓度为0.05mg/kg。结论该方法简便、快速、准确,可为叶菌唑在小麦中的残留分析提供技术依据。
OBJECTIVE To establish a method for the determination of meconazole residues in soil, wheat flour and wheat plants by solid-phase extraction and high performance liquid chromatography. Methods Soil, wheat flour and wheat samples were extracted with acetonitrile, purified by Silica-SPE SPE cartridge, separated on Eclicpse Plus C_ (18) column and detected by high performance liquid chromatography (HPLC). Results There was a good linear relationship between metconazole and 0.1 ~ 10 mg / L. The standard curve equation was Y = 37.395X-1.0902 with a correlation coefficient of 0.9999. The average recoveries of metconazole in soil, wheat flour and wheat plants ranged from 87.2% to 100.9% at spiking levels of 0.05-2.0 mg / kg with relative standard deviations of 0.2% -8.7%. Metconazole the lowest detection concentration of 0.05mg / kg. Conclusion The method is simple, rapid and accurate and can provide technical basis for the residual analysis of metconazole in wheat.