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目的 探讨散发性结直肠癌中微卫星不稳定性 (MIN)发生与 p5 3蛋白表达的相关性及其意义。方法 采用微卫星 DNA- PCR-银染色法检测 6 7例散发性结直肠癌 4条染色体上 6个微卫星位点的 MIN;应用 SABC方法检测 p5 3蛋白表达。结果 以 2个或 2个以上位点有 MIN定义为复制误差阳性 (RER+) ,RER+率 41.8% (2 8/6 7)。 5 0例 p5 3蛋白表达阳性 (5 0 / 6 7,74.6 % )。RER+组 p5 3蛋白阳性率 89.3% (2 5 / 2 8) ,RER- 组 p5 3蛋白阳性率6 4.1% (2 5 / 39) ,两组间差异有显著性 (P<0 .0 5 )。结论 p5 3基因突变以及误配修复系统缺陷造成的基因组不稳定性是散发性结直肠癌演进过程中重要的分子遗传学改变 ,两者间可能存在一定的关系。
Objective To investigate the correlation between the occurrence of microsatellite instability (MIN) and the expression of p53 protein in sporadic colorectal cancer and its significance. Methods The microsatellite DNA-PCR-silver staining method was used to detect the MIN of 6 microsatellite loci on 6 chromosomes of 4 sporadic colorectal cancers. The expression of p53 protein was detected by SABC method. Results There were 2 or more sites with a MIN defined as positive replication error (RER+) with a RER+ rate of 41.8% (2 8/67). The expression of p53 protein was positive in 50 cases (50/647,74.6%). The positive rate of p53 protein in RER+ group was 89.3% (2 5 / 28), and the positive rate of p5 3 protein in RER- group was 6 4.1% (2 5 / 39). There was significant difference between the two groups (P<0.05). . Conclusion The genomic instability caused by p53 gene mutation and defects in mismatch repair system are important molecular genetic changes in the evolution of sporadic colorectal cancer. There may be a certain relationship between them.