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５０例胃癌和２０例非癌对照的胃手术切除标本，应用免疫组化和图像分析技术，检测癌基因蛋白ｐ~（５３）、ｃ－ｅｒｂＢ－２和ＰＣＮＡ的表达。结果显示胃癌ｐ~（５３）异常表达率为５８％（２９／５０），ｃ－ｅｒｂＢ－２过度表达率为３４％（１７／５０），ＰＣＮＡ为＞５０％的高增殖指数值；对照组癌基因呈阴性表达，ＰＣＮＡ＜２０％，差别有显著性（Ｐ＜０．０５），表明胃癌发生与该２种癌基因异常表达有一定关系。２８例癌旁粘膜研究显示腺体重度异型增生和大肠型肠化者ｐ~（５３）表达率８５％和７８％，而轻度异型增生和小肠型肠化ｐ~（５３）呈阴性反应（Ｐ＜０．０２）。对传统的大肠型和小肠型肠化上皮依据ｐ~（５３）蛋白表达情况，可进一步分析其性质，即ｐ~（５３）突变型的大肠型肠化上皮具有癌变潜能，归属癌前病变；ｐ~（５３）野生型的小肠型肠化上皮不是癌前病变。ｐ~（５３）蛋白表达对胃早癌及癌前病变鉴别诊断有重要临床意义。
Gastric resection specimens of 50 cases of gastric cancer and 20 cases of non-cancer controls were examined by immunohistochemistry and image analysis techniques to detect the expression of oncogene proteins p53, c-erbB-2, and PCNA. The results showed that the abnormal expression rate of p53 in gastric cancer was 58% (29/50), the over-expression rate of c-erbB-2 was 34% (17/50), and the PCNA was higher than 50%; the control group Oncogene negative expression, PCNA <20%, the difference was significant (P <0.05), indicating that the occurrence of gastric cancer has a certain relationship with the abnormal expression of these two oncogenes. In 28 cases of paracancerous mucosa, the p53 expression rate was 85% and 78% in glandular dysplasia and colonic intestinal metaplasia, while mild dysplasia and p53 in the small intestine were negative. P<0.02). Based on the expression of p53 protein in the colonic and small intestine intestinal epithelium, we can further analyze its nature, that is, the p53 mutant colonic intestinal metaplasia has cancer potential and belongs to precancerous lesion; The p53 wild-type intestinal metaplasia is not a precancerous lesion. The expression of p53 protein has important clinical significance in the differential diagnosis of gastric cancer and precancerous lesions.