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目的 :建立丹参及其亲缘植物中丹参酮类与丹酚酸类成分同时检测的含量测定方法 ,为丹参的质量控制及临床替代用药提供科学依据。方法 :采用高效液相色谱法测定 9种主要成分的含量。色谱柱为ShimadzuCLC ODS柱 (15 0mm× 6mm ,5 μm) ,流动相为乙腈、0 5 %甲酸水溶液梯度洗脱 ,流速为 0 8mL·min-1,检测波长 0～ 2 5min为 2 81nm ,2 5min后为 2 5 4nm。结果 :9种成分在线性范围内均呈现良好的线性 (r>0 999)。结论 :丹参及其亲缘植物丹参酮类、丹酚酸类的含量差异显著 ,同种不同产地的样品之间也存在明显差异 ,丹参的质量控制是一个亟待解决的问题 ;云南鼠尾、红根草中几乎未检出丹参酮类成分 ,不宜作为丹参使用
Objective : To establish a method for the simultaneous determination of tanshinones and salvianolic acids in Salvia miltiorrhiza and its related plants, providing scientific basis for the quality control of Salvia miltiorrhiza and its clinical alternative use. Method : The content of 9 main components was determined by HPLC. The column was a ShimadzuCLC ODS column (15 mm × 6 mm, 5 μm). The mobile phase was acetonitrile and a 0.5% aqueous formic acid solution. The flow rate was 0 8 mL·min-1. The detection wavelength was 0 to 25 min, which was 2 81 nm. 2 After 5 min, it was 2 5 4 nm. RESULTS : All nine components showed good linearity in the linear range (r>0 999). Conclusion: There are significant differences in the contents of tanshinones and salvianolic acids in Salvia miltiorrhiza and its relatives, and there are also significant differences between samples of the same species in different producing areas. The quality control of Salvia miltiorrhiza is a problem to be solved; Yunnan rat tail and red root grass Almost no tanshinones were detected in the product and should not be used as Salvia miltiorrhiza.