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建立了测定大鼠血浆中西瑞香素浓度的高效液相色谱法,并对其进行方法学确证。以蛋白沉淀法和液液萃取法对大鼠血浆中西瑞香素进行提取。采用Diamonsil C_(18)柱(200mm×4.6mm,5μm),流动相为甲醇-20mmol/L醋酸铵(醋酸调pH至3.2,42:58,v/v),流速1.0mL/min。检测波长345nm,柱温40℃。西瑞香素在0.020-2.00μg/mL浓度范围内线性关系良好(r>0.9900),定量下限0.020μg/mL。日内和日间精密度(RSD)为5.0%-10.6%,准确度(RE)为±(1.2%-2.5%)。所建立的方法成功应用于腹腔注射西瑞香素在大鼠体内的药代动力学研究。
A high performance liquid chromatography (HPLC) method for the determination of Seraphosin in rat plasma was established, and the method was validated. Sera from rats were extracted by protein precipitation and liquid-liquid extraction. Diamonsil C_ (18) column (200mm × 4.6mm, 5μm) was used. The mobile phase consisted of methanol-20mmol / L ammonium acetate (acetic acid adjusted to pH 3.2, 42:58, v / v) and the flow rate was 1.0mL / min. Detection wavelength 345nm, column temperature 40 ℃. Ceria diterpene had a good linearity (r> 0.9900) at a concentration range of 0.020-2.00μg / mL with a lower limit of quantification of 0.020μg / mL. The intra- and inter-day precision (RSD) was 5.0% -10.6% and the accuracy (RE) was ± (1.2% -2.5%). The established method was successfully applied to study the pharmacokinetics of silybin in rats by intraperitoneal injection.