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本文采用高效液相色谱法，测定中药制剂双黄连粉针中黄芩甙和绿原酸含量。流动相分别是0．1mol／L磷酸二氢钾：乙酸：四氢呋喃（100：10：19）和甲醇-10％磷酸缓冲液（10：90），流速分别是0．8和1ml／min；色谱柱ERC-ODS-1161（6×100mm），柱温55℃；黄芩甙以电化学为检测器，检测电压是＋650mv；绿原酸以紫外为检测器，检测波长274nm；结果：该方法的最小检测浓度分别为0．025mg／L和0．1mg／L，平均回收率分别为100．1％和100．4％，重现性好。本方法测定快速、简便、准确，适用于含黄芩甙和绿原酸的中药制剂的含量测定。
In this paper, high performance liquid chromatography was used to determine the content of Astragalus membranaceus and chlorogenic acid in Shuanghuanglian injection. The mobile phases were 0.1 mol/L potassium dihydrogen phosphate:acetic acid:tetrahydrofuran (100:10:19) and methanol-10% phosphoric acid buffer (10:90), and the flow rates were 0.8 and 1 ml/min, respectively; Column ERC-ODS-1161 (6×100mm), column temperature 55°C; Astragalus using electrochemical detector, detection voltage is +650mv; Chlorogenic acid UV detector, detection wavelength 274nm; Result: The method is the smallest The detection concentrations were 0.025 mg/L and 0.1 mg/L, respectively. The average recoveries were 100.1% and 100.4%, respectively. The reproducibility was good. The method is rapid, simple, accurate, and suitable for the determination of traditional Chinese medicine preparations containing astragalus and chlorogenic acid.