通过建构质粒在酵母菌(saceharomyces cerevisiae)内直接合成干扰素(IFN)α1,α2和γ,这种干扰素表达基因包括含有分泌信号编码序列能在培养液中分泌活性干扰素。从酵母细胞培养液内分离获得的大部分IFN α1,α2,与天然成熟干扰素具有相同的氨基末端,揭示这种移动的信号序列类似人细胞的信号序列.以上结果说明,低等真核生 Interferon (IFN) α1, α2 and γ are directly synthesized by constructing plasmids in yeast (saceharomyces cerevisiae). Such interferon expression genes include secreted signal coding sequences capable of secreting active interferons in the culture medium. Most IFNα1, α2, isolated from yeast cell culture fluid, have the same amino terminus as native mature interferon, revealing that the mobile signal sequence resembles the human cell signal sequence.The above results indicate that the lower eukaryotic