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目的:探索氢氧化镁对BSA微球体外释放的影响,优化BSA微球的制备工艺。方法:通过水包油包固复乳法制备BSA-PLGA微球。先将BSA与葡聚糖制备成玻璃体颗粒,再将玻璃体颗粒与氢氧化镁包裹进PLGA中,制备成缓释微球。在扫描电镜下观察其形态。然后用Micro BCA法测定其包封率和载药量,并考察其体外释放行为。结果:所制得的微球粒径约60μm,呈较好的球形。添加氢氧化镁后,BSA微球的包封率和载药量都有显著提高。不同含量的氢氧化镁对BSA微球的包封率和载药量影响也不同。在体外释放过程中,载有氢氧化镁的微球14天累积释放量为(85.10±2.67)%,而对照组不到80%。结论:通过调整氢氧化镁的量,可以制得形态完整,大小均匀,突释较小的BSA微球。
Objective: To explore the effect of magnesium hydroxide on the release of BSA microspheres in vitro and to optimize the preparation process of BSA microspheres. Methods: BSA-PLGA microspheres were prepared by means of oil-in-water-encapsulated and double-emulsion method. BSA and dextran were first prepared into vitreous particles, and then the vitreous particles and magnesium hydroxide wrapped into the PLGA to prepare sustained-release microspheres. The morphology was observed under a scanning electron microscope. Then the micro BCA method was used to determine the entrapment efficiency and drug loading, and to investigate its in vitro release behavior. Results: The prepared microspheres had a diameter of about 60 μm and a good spherical shape. After adding magnesium hydroxide, the encapsulation efficiency and drug loading of BSA microspheres increased significantly. Different content of magnesium hydroxide on BSA microspheres entrapment efficiency and drug loading are also different. During in vitro release, the cumulative release of microspheres loaded with magnesium hydroxide was (85.10 ± 2.67)% in 14 days compared to less than 80% in the control group. Conclusion: By adjusting the amount of magnesium hydroxide, BSA microspheres with complete shape, uniform size and small burst release can be obtained.