微流控二维芯片电泳技术因其具有分离速度快、易于微型化和自动化、接口处死体积小、可极大地改善峰容量和分辨率等优点,而在复杂的蛋白质组学研究中展现出了巨大的潜力。文中针对蛋白质微流控二维芯片电泳分离原理、芯片的设计和制作、检测技术、分离分析条件的优化等进行了综述,按二维接口模式分类讨论了微流控二维芯片电泳技术在蛋白质分离分析中的研究现状和最新进展,并就微流控二维芯片技术在蛋白质组学研究中的应用前景进行了展望。 Microfluidic two-dimensional chip electrophoresis technology because of its separation speed, easy miniaturization and automation, the interface sacrificial volume, can greatly improve the peak capacity and resolution advantages, but in complex proteomics studies have shown huge potential. In this paper, the principle of two-dimensional electrophoresis separation of protein microfluidic chip, the design and fabrication of the chip, the detection technology and the optimization of the separation and analysis conditions were reviewed. According to the two-dimensional interface model, Separation and analysis of research status and the latest progress, and microfluidic two-dimensional chip technology in proteomics research prospects.