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本文用大孔网高分子载体对氨基酰化酶进行固定化,并系统地研究了温度、pH值、离子浓度、底物浓度、激活离子及蛋白质变性剂对固定化酶活力的影响。由于载体的物理性质和功能基较适宜,因而吸附蛋白质的容量大,使固定化酶的活力和活力回收率都高。用固定化酶柱对D,L—蛋氨酸进行连续拆分,酶柱使用周期长,稳定性高。拆分的产物用离子交换法进行分离与精制后,可制得纯度高,产率理想的L—蛋氨酸盐酸盐。
In this paper, macroporous macromolecule carriers were used to immobilize aminoacylase. The effects of temperature, pH value, ionic strength, substrate concentration, activated ions and protein denaturant on immobilized enzyme activity were systematically investigated. Due to the physical properties of the carrier and functional base is more appropriate, so the adsorption capacity of the protein, the immobilized enzyme activity and vitality recovery rate is high. With immobilized enzyme column on D, L-methionine for continuous resolution, the use of enzyme column long cycle, high stability. After the separated product is separated and purified by ion exchange method, L-methionine hydrochloride with high purity and high yield can be obtained.