高效液相色谱法可以将食物中不同生理活性的维生素E及维生素A(V_E和V_A)分离,用苯并[e]芘为内标准定量。分离柱为C_(18)反相柱,流动相为甲醇:水(98:2),流速1.8ml/min,检测波长为300nm。 食物皂化后用乙醚提取,提取液蒸干后加流相使维生素溶入,离心,取样品提取清液注 High performance liquid chromatography (HPLC) can separate vitamin E and vitamin A (V_E and V_A) from different physiologically active substances in foods and quantify them with benzo [e] pyrene as an internal standard. The separation column was C_ (18) reversed-phase column. The mobile phase was methanol: water (98: 2), the flow rate was 1.8ml / min and the detection wavelength was 300nm. Saponification of food after extraction with ether, the extract was evaporated to dryness and add the flow of the vitamin solution, centrifuged, the sample was extracted clear note