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目的建立一种同时测定食品中6种大豆异黄酮[大豆苷元(daidzein)、黄豆黄素(glycitein)、染料木素(genistein)、黄豆黄苷(glycitin)、大豆苷(daidzin)和染料木苷(genistin)]的快速溶剂萃取-高效液相色谱检测方法。方法样品经快速溶剂萃取仪萃取,采用phenomenex LunaC18柱,以0.05%三氟乙酸(TFA)溶液和乙腈为流动相梯度洗脱,二极管阵列检测器检测。结果连续7次测定6种大豆异黄酮,相对标准偏差为1.4%～5.9%。加标回收率82%～112%。在0.01～10μg/ml范围内线性关系良好、最低检出限10μg/kg。结论该方法操作简便,萃取液共存物质对测定方法干扰较小,仪器测定方法准确。适用于大豆异黄酮的检测。
OBJECTIVE To establish a method for the simultaneous determination of six soy isoflavones (daidzein, glycitein, genistein, glycitin, daidzin and dyestuff in food) Genistin] rapid solvent extraction - high performance liquid chromatography detection method. Methods The samples were extracted by fast solvent extraction. Phenomenex Luna C18 column was used for the mobile phase gradient elution with 0.05% trifluoroacetic acid (TFA) and acetonitrile. Detected by diode array detector. Results Seven kinds of soy isoflavones were determined seven times in succession, with relative standard deviations of 1.4% -5.9%. Spike recovery 82% ~ 112%. The linearity was good in the range of 0.01 ~ 10μg / ml with the lowest detection limit of 10μg / kg. Conclusion The method is simple and easy to operate. The coexisting substances in the extract have less interference with the determination method and the instrument is accurate. Suitable for the detection of soy isoflavones.