China has accumulated massive fine grained copper mine tailings stocks because of the past mining activities in this area. The tailings contain a variety of heavy metals, and the mass percent of Cu, which is one of the main contaminants in tailings, is up to 0.2601% (analysis by XRF). The Cu can pollute soil and groundwater by rain leaching in the form of Cu(Ⅱ), furthermore ,the fine grained copper-ore-tailings can contaminant larger area by wind for its small granularity ( < 74 μm). The main cause of weathering of mine tailings is due to oxidative dissolution of sulfides. Microorganisms, such as Acidithiobacillus ferrooxidans, play an important role in weathering. These bacteria attach to exposed to mineral surfaces by excreting extracellular polymers and oxidize the sulfide mineral. Some of these bacteria also oxidize Fe2+ to Fe3+ which can chemically oxidize sulfide minerals. These reactions produce voluminous quantities of acid mine drainage and heavy metals which are harmful to the environment and human healthy. This study aims at finding the weathering effects of A. ferrooxidans to Cu(II) pollution of fine grained copper mine tailings, and our experiment applied indigenous A. ferrooxidans FJ-01 to leach the tailings. The optimum test parameters were obtained using shaking flask experiment and SEM observation under the following experimental conditions: 39 days residence time, pulp density 1%-15% (1%, 5% and 15%), 30℃, 120 rpm, pH between 1-3 and redox potential between 400-650 mV. The test results show that the leaching rate of Cu reached 43.1% when the pulp density was 1% after 33 days and kept invariant till the end of the test. In addition, the leaching rate of Cu will decrease as the increase of pulp density, and the maximum rate of 15% pulp density was only 12.5%. From the SEM, it can be seen that the fine grain of tailings flocculated to conglobation under the action of bacterial leaching. China has accumulated massive fine grained copper mine tailings stocks because of the past mining activities in this area. The tailings contain a variety of heavy metals, and the mass percent of Cu, which is one of the main contaminants in tailings, is up to 0.2601 % (analysis by XRF). The Cu can pollute soil and groundwater by rain leaching in the form of Cu (II), furthermore, the fine grained copper-ore-tailings can contaminant larger area by wind for its small granularity (<74 μm The main cause of weathering of mine tailings is due to oxidative dissolution of sulfides. Microorganisms, such as Acidithiobacillus ferrooxidans, play an important role in weathering. These bacteria attach to exposed mineral surfaces by excreting extracellular polymers and oxidize the sulfide mineral. Some of these bacteria also oxidize Fe2 + to Fe3 + which can chemically oxidize sulfide minerals. These reactions produce voluminous quantities of acid mine drainage and heavy metals which are harmful to the environment and human healthy. This study aims at finding the weathering effects of A. ferrooxidans to Cu (II) pollution of fine grained copper mine tailings, and our experiment applied indigenous A. ferrooxidans FJ-01 to leach the tailings. The optimum test parameters were obtained in shaking flask experiment and SEM observation under the following experimental conditions: 39 days residence time, pulp density 1% -15% (1%, 5% and 15%), 30 ° C, 120 rpm, pH between 1-3 and redox potential between 400-650 mV. The test results show that the leaching rate of Cu reached 43.1% when the pulp density was 1% after 33 days and kept invariant till the end of the test. In addition, the leaching rate of Cu will decrease as the increase of pulp density, and the maximum rate of 15% pulp density was only 12.5%. From the SEM, it can be seen that the fine grain of tailings flocculated to conglobation under the action of bacterial leaching.