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目的观察降脂平肝汤含药血清对大鼠3T3-L1脂肪细胞胰岛素受体底物1(IRS-1)mRNA和过氧化物酶增殖体激活受体(PPAR)-γmRNA表达的影响,探讨其对脂肪细胞胰岛素抵抗影响的可能机制。方法选择Wistar大鼠16只,诱导分化3T3-L1脂肪前体细胞为成熟的脂肪细胞并以肿瘤坏死因子α(TNFα)诱导建立脂肪细胞的胰岛素抵抗模型,取诱导成功的胰岛素抵抗模型细胞分为对照组(基础培养液加入20%大鼠空白血清)、模型组(基础培养液加入20%大鼠空白血清和20ng/mlTNF-α)、罗格列酮组(基础培养液加入20%大鼠含药血清和20ng/mlTNF-α)、降脂平肝汤组(基础培养液加入20%大鼠含药血清和20ng/mlTNF-α)每组4只,培养48h,观察细胞IRS-1mRNA和PPAR-γmRNA表达水平的变化。结果模型组IRS-1mRNA和PPAR-γmRNA表达明显降低,与对照组比较差异有统计学意义(P<0.05);罗格列酮组与降脂平肝汤组IRS-1mRNA和PPAR-γmRNA表达均明显增高,与模型组比较差异有统计学意义(P<0.05)。结论降脂平肝汤可能通过增强大鼠IRS-1、PPAR-γ基因的表达起到抑制脂肪细胞胰岛素抵抗的作用。
Objective To investigate the effects of Jiangzhipinggan decoction serum on insulin receptor substrate 1 (IRS-1) mRNA and peroxisome proliferator activated receptor (PPAR) -γ mRNA expression in rat 3T3-L1 adipocytes Its possible mechanism of affecting insulin resistance in adipocytes. Methods Totally 16 Wistar rats were used to induce differentiation of 3T3-L1 adipose precursor cells into mature adipocytes and insulin-like growth factor (TNF) -induced adipocyte induction. Cells were induced into successful insulin resistance model In the control group (20% rat blank serum was added to the basal medium), the model group (20% rat serum and 20 ng / ml TNF- α in the basal medium), the rosiglitazone group (20% Α), Jiangzhi Pinggan Decoction (20% rat serum and 20ng / ml TNF-α in the basal medium) were cultured in each group for 48 hours. The mRNA and protein levels of IRS-1 mRNA and protein PPAR-γmRNA expression level changes. Results The expressions of IRS-1mRNA and PPAR-γmRNA in model group were significantly lower than those in control group (P <0.05). The expressions of IRS-1mRNA and PPAR-γmRNA in rosiglitazone group and Jiangzhipinggan group Was significantly higher than that of the model group (P <0.05). Conclusion Jiangzhi Pinggan Decoction may inhibit the insulin resistance of adipocytes by enhancing the expression of IRS-1 and PPAR-γ in rats.