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目的观察1型糖尿病大鼠血清胰岛素和骨钙素水平,骨组织Runx2和Osterix(OSX)基因转录水平及唑来膦酸的干预作用。方法 Wistar雌性大鼠170只,随机分为正常对照组(n=40)和糖尿病模型组(n=130)。糖尿病模型组一次性腹腔注射链脲佐菌素(60 mg/kg)制作1型糖尿病模型,随机选取120只分为模型组、预防组和治疗组,每组40只。预防组和治疗组分别在造模成功和造模成功2周后静脉给予唑来膦酸(0.1 mg/kg)。ELISA法检测血清胰岛素和骨钙素水平;RT-PCR法检测右侧股骨Runx2和OSX mRNA表达水平。结果模型组、预防组和治疗组与正常对照组相比,血清胰岛素水平显著下降(P<0.05)。骨钙素水平模型组显著低于正常对照组(P<0.05),预防组高于正常对照组和模型组(P<0.05),治疗组在12周高于预防组(P<0.05)。模型组骨组织Runx2和OSX mRNA表达水平与正常对照组相比显著降低(P<0.05),预防组和治疗组表达均明显高于正常对照组和模型组(P<0.05),预防组表达上调程度高于治疗组。结论在1型糖尿病大鼠模型中,骨组织Runx2和OSX mRNA表达水平显著下降;预防性应用唑来膦酸可逆转Runx2和OSX mRNA表达的下降,促进骨形成。
Objective To observe the levels of serum insulin and osteocalcin in type 1 diabetic rats, the transcriptional level of Runx2 and Osterix (OSX) in bone and the intervention of zoledronic acid. Methods 170 Wistar female rats were randomly divided into normal control group (n = 40) and diabetic model group (n = 130). Type 1 diabetes model was made by intraperitoneal injection of streptozotocin (60 mg / kg) in diabetes mellitus model group, 120 rats were randomly divided into model group, prevention group and treatment group, with 40 rats in each group. Prevention and treatment groups were given zoledronic acid (0.1 mg / kg) intravenously 2 weeks after successful modeling and successful modeling. Serum insulin and osteocalcin levels were measured by ELISA. The expression of Runx2 and OSX mRNA in right femur was detected by RT-PCR. Results Compared with the normal control group, serum insulin levels in the model group, prophylaxis group and treatment group were significantly decreased (P <0.05). The level of osteocalcin in the model group was significantly lower than that in the normal control group (P <0.05). The level of the preventive group was higher than that of the normal control group and the model group (P <0.05). The treatment group was higher than the prevention group at 12 weeks (P <0.05). The expressions of Runx2 and OSX mRNA in model group were significantly lower than those in normal control group (P <0.05), and were significantly higher in prevention group and treatment group than those in normal control group and model group (P <0.05) Degree higher than the treatment group. CONCLUSIONS: The expression of Runx2 and OSX mRNA in bone tissue of type 1 diabetic rats decreased significantly. Preventive zoledronic acid treatment could reverse the decrease of Runx2 and OSX mRNA expression and promote bone formation.