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在合成和表征了 4种具有较强生物活性双过氧钒配合物K3 [VO(O2 ) 2 (ox) ]·2H2 O ,Na[VO(O2 ) 2 (bipy) ]·5H2 O ,K[VO(O2 ) 2 (phen) ]·3H2 O和K2 [VO(O2 ) 2 (pic) ]·2H2 O [分别缩写为pV(ox) ,pV(bipy) ,pV(phen)和pV(pic) ,其中ox为草酸根 ,bipy为 2 ,2′ 联吡啶 ,phen为邻菲咯啉 ,pic为 2 羧酸吡啶负离子 ]的基础上 ,利用多种NMR技术和电喷雾质谱 (ESI MS)研究了这 4种含有机配体双过氧钒配合物与组氨酸 (His)在溶液中的相互作用以及反应物浓度、时间、pH等对相互作用体系的影响 .51VNMR跟踪监测的结果表明 :双过氧钒配合物pV(ox)和pV(pic)与His在中性水溶液中存在强配位相互作用 ,而pV(bipy)和pV(phen)与His在中性水溶液中无明显作用 .我们还利用谱学方法确定了pV(ox)和His相互作用后所生成产物是pV(ox)分别与His咪唑基上的 3 N和 1 N配位的一对异构体
Four kinds of complexes with strong biologically active diperoxides such as K3 [VO (O2) 2 (ox)] · 2H2O, Na [VO (O2) (Phen)] 2H2 O and K2 [VO (O2) 2 (pic)] • 2H20 [abbreviated as pV (ox), pV (bipy) , Where ox is oxalate, bipy is 2, 2 ’bipyridyl, phen is phenanthroline and pic is 2-carboxypyridine anion], various NMR techniques and electrospray ionization mass spectrometry (ESI MS) The interactions between these four organic ligands such as histidine (His) and histidine (His) in solution and the effect of reactant concentration, time and pH on the interaction system were investigated.The results of 51V NMR trace monitoring showed that bis PV (ox) and pV (pic) had a strong coordination interaction with His in neutral aqueous solution, whereas pV (bipy) and pV (phen) did not show significant effect in neutral aqueous solution. Spectroscopic methods were also used to confirm that the product of pV (ox) and His interaction is a pair of isomers in which pV (ox) is coordinated to 3N and 1N on His imidazolyl, respectively