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目的:探讨宫颈鳞状细胞癌中PAI-1、TGF-β1表达意义及其与HPV16感染的关系。方法:免疫组化检测PAI-1、TGF-β1在63例宫颈鳞状细胞癌、15例正常宫颈中的表达,HPV16 DNA的检测应用原位杂交法。结果:HPV16、PAI-1、TGF-β1在宫颈鳞状细胞癌中的阳性率分别为46.03%、68.25%、55.56%;HPV16、PAI-1、TGF-β1在正常宫颈中的阳性率分别为6.67%、0.00%、13.33%。HPV16、PAI-1、TGF-β1在两组间的表达差异有统计学意义(P<0.05)。PAI-1、TGF-β1在子宫颈癌HPV阳性和阴性组表达差异无统计学意义(P>0.05),TGF-β1表达与PAI-1有关(P<0.05)。结论:宫颈鳞状细胞癌中PAI-1、TGF-β1的阳性表达可能与其发生、发展相关,促进其浸润与转移,PAI-1、TGF-β1的表达与HPV16感染无关。
Objective: To investigate the expression of PAI-1 and TGF-β1 in cervical squamous cell carcinoma and its relationship with HPV16 infection. Methods: The expressions of PAI-1 and TGF-β1 in 63 cases of cervical squamous cell carcinoma and 15 cases of normal cervix were detected by immunohistochemistry. The detection of HPV16 DNA was detected by in situ hybridization. Results The positive rates of HPV16, PAI-1 and TGF-β1 in cervical squamous cell carcinoma were 46.03%, 68.25% and 55.56% respectively. The positive rates of HPV16, PAI-1 and TGF- 6.67%, 0.00%, 13.33%. The expression of HPV16, PAI-1 and TGF-β1 in the two groups had statistical significance (P <0.05). There was no significant difference in the expression of PAI-1 and TGF-β1 between HPV positive and negative groups (P> 0.05). The expression of TGF-β1 was correlated with PAI-1 (P <0.05). Conclusion: The positive expression of PAI-1 and TGF-β1 in squamous cell carcinoma of cervix may be related to the occurrence and development of squamous cell carcinoma and promote the infiltration and metastasis of PAI-1 and TGF-β1.