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在转录折叠条件下,丁型肝炎病毒(HDV)的自剪切活性的发挥受到转录过程中形成的中间态结构、转录速率、突变位点等的影响.本文采用转录折叠动力学方法研究了HDV的天然(wild-type,wt)序列在不同转录速率下的转录折叠动力学行为,并分析了G11C突变对转录过程的影响.研究结果表明,虽然HDV的天然序列和G11C突变序列的转录折叠过程都存在快慢两条路径,但是对于HDV的天然序列,增大转录速率可以降低其慢速折叠路径上的RNA占据几率,而对于G11C突变序列,增大转录速率反而使得更多的RNA经过慢速路径形成天然态结构,并且在转录完全结束以后,HDV的天然序列要比G11C突变序列更快速地形成天然态结构.
Under transcriptional folding conditions, the self-shearing activity of hepatitis B virus (HDV) is affected by the intermediate structure, transcription rate and site of mutation in the process of transcription.In this paper, the transcriptional folding kinetics was used to study the effects of HDV The transcriptional folding dynamics of wild-type (wt) sequences at different transcriptional rates was analyzed and the effect of G11C mutation on the transcriptional process was analyzed.The results showed that although the natural folding of HDV and the transcriptional folding process of G11C mutation There are two fast and slow pathways, but for the native sequence of HDV, increasing the transcription rate reduces the chances of RNA on its slow folding path, whereas for the G11C mutation sequence, increasing the transcription rate causes more RNA to slow down The pathways form a natural-state structure, and after the transcription ends completely, the native sequence of HDV forms the native state structure more rapidly than the G11C mutation sequence.