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目的研究盐度和温度调节副溶血弧菌(Vibrio parahaemolyticus)的运动能力。方法将副溶血弧菌接种于不同盐度(0.5%、1.0%、2.0%和4.0%)的爬动和泳动平板上,37℃静置培养4.5 h后,通过比较不同盐度条件菌苔直径的差异来判定盐度对运动能力的影响。接种副溶血弧菌至含2.0%盐的爬动和泳动平板上,并分别置于37℃和26℃静止培养4.5 h后,通过比较不同温度下菌苔直径的差异来研究温度对运动能力的影响。结果与结论副溶血弧菌爬动不受盐度的影响,而其泳动与盐度呈正相关,2.0%时达到极值,4.0%时稍微下降;与26℃培养的结果相比,37℃培养时爬动和泳动均显著增强。这些结果表明盐度和温度能够调节副溶血弧菌的运动能力。
Objective To study the salinity and temperature regulation of the movement ability of Vibrio parahaemolyticus. Methods Vibrio parahaemolyticus was inoculated on crawling and swimming plates with different salinities (0.5%, 1.0%, 2.0% and 4.0%). After incubation at 37 ℃ for 4.5 h, Diameter differences to determine the impact of salinity on exercise capacity. Vibrio parahaemolyticus was inoculated on a slide and plate with 2.0% saline and placed in stationary culture at 37 ° C and 26 ° C for 4.5 h, respectively. After comparing the differences in the diameters of lawn moss at different temperatures, the effects of temperature on exercise capacity Impact. RESULTS AND CONCLUSIONS Vibrio parahaemolyticus was not affected by salinity, and its migration was positively correlated with salinity, reaching the peak at 2.0% and slightly decreasing at 4.0%. Compared with the results at 26 ℃, Creeping and swimming motility were significantly enhanced when cultured. These results indicate that salinity and temperature can regulate the movement ability of V. parahaemolyticus.