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目的:分离获得西瓜枯萎病高效拮抗菌,为研究西瓜枯萎病高效拮抗菌的拮抗机制奠定基础。方法:用碾碎法从新疆有毒植物麻(Urtica cannabina L.)、亚洲薄荷(Mentha asiatica Boris.)、阿尔泰藜芦(Veratrum lobelianum Bernh.)中分离内生菌,并用琼脂扩散法筛选出对西瓜枯萎病具有较强抗性的内生菌XJUL-12,通过PCR方法扩增XJUL-12的16S rDNA,并与GeneBank中已鉴定菌的16S rDNA序列对比,用N-J方法构建XJUL-12进化树,用Bootstraping法对其评估,同时结合其形态特征、生理生化检测、G+C mol%含量对XJUL-12进行鉴定。结果:XJUL-12的16S rDNA序列与Bacillus subtilisstrain CGMCC1869同源性为99%,G+C mol%含量为46.72mol%。结论:筛选获得的内生菌XJUL-12对西瓜枯萎病具有较强抗性,并将XJUL-12鉴定为枯草芽孢杆菌。
OBJECTIVE: To isolate and obtain effective antagonistic bacteria against watery fusarium wilt and lay a foundation for studying the antagonistic mechanism of antagonistic bacteria against water wilt disease. Methods: Endophytic bacteria were isolated from Xinjiang Urtica cannabina L., Mentha asiatica Boris. And Veratrum lobelianum Bernh. By using agar diffusion method, XJUL-12 was amplified by PCR. The 16S rDNA of XJUL-12 was amplified by PCR and compared with 16S rDNA sequences of identified strains in GeneBank. The XJUL-12 phylogenetic tree was constructed by NJ method. Bootstraping method was used to evaluate the XJUL-12, and its morphological characteristics, physiological and biochemical tests and G + C mol% content were also evaluated. Results: The 16S rDNA sequence of XJUL-12 shared 99% homology with Bacillus subtilisstrain CGMCC1869 and the content of G + C mol% was 46.72mol%. Conclusion: The selected endophytic strain XJUL-12 has strong resistance to watermelon blight and identified XJUL-12 as Bacillus subtilis.