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本文采用扫集-胶束毛细管电动色谱法(Sweeping-MEKC)分离测定急支糖浆中的阿魏酸、原儿茶醛和原儿茶酸。采用未涂层熔融石英毛细管(50cm×50μm,有效柱长36cm),环境温度25℃,缓冲体系为20mmol/L NaH2PO4+80mmol/L十二烷基磺酸钠(SDS)+12.5%乙腈(V/V)(pH=2.2),紫外检测波长225nm,运行分离电压-20kV,进样时间60s,达到最佳的分离效果。在优化条件下,阿魏酸、原儿茶醛和原儿茶酸均在15min内出峰,峰面积的相对标准偏差(RSD)均小于5%,检出限分别为109.95μg/L、88.48μg/L和15.96μg/L。
In this paper, sweeping-micellar capillary electrokinetic chromatography (Sweeping-MEKC) was used to separate the ferulic acid, protocatechuic aldehyde and protocatechuic acid in the acute syrup. Uncoated fused silica capillary (50cm × 50μm, effective column length 36cm) was used, the temperature was 25 ℃, buffer system was 20mmol / L NaH2PO4 + 80mmol / L sodium dodecyl sulfate (SDS) /V)(pH=2.2), UV detection wavelength of 225nm, running separation voltage -20kV, injection time 60s, to achieve the best separation. Under optimized conditions, the ferulic acid, protocatechuic aldehyde and protocatechuic acid all peaked within 15 min, and the relative standard deviations (RSDs) of peak area were all less than 5%. The detection limits were 109.95μg / L, 88.48 μg / L and 15.96 μg / L.