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目的观察大鼠血肿周围脑组织IL-1β蛋白表达的变化规律,并探讨抑肽酶的脑保护作用。方法尾状核注射胶原酶诱导大鼠脑出血(ICH)模型,分别用免疫组化法和ELISA法测定IL-1β的蛋白表达水平,参照Berderson’s法进行神经功能缺损评分。结果模型组血肿周围脑组织的IL-1β阳性细胞数和蛋白含量在6 h即有增加,48 h左右达高峰,而抑肽酶组相应各时间点的阳性表达明显减少,尤以48、72 h较为明显;模型组与抑肽酶组在61、2 h的神经功能缺损评分无显著性差异,其余各时间点均有显著性差异(P<0.01)。结论IL-1β参与了脑出血后的继发性脑损伤,抑肽酶能够明显减轻脑出血后的IL-1β表达,从而发挥脑保护作用。
Objective To observe the changes of IL-1β protein expression in the perihematomal brain tissue of rats and to explore the protective effect of aprotinin. Methods The rat model of intracerebral hemorrhage (ICH) was induced by injection of collagenase into the caudate nucleus. The protein expression of IL-1β was determined by immunohistochemistry and ELISA respectively. The neurological deficit score was determined by Berderson’s method. Results The number of IL-1β positive cells and the content of protein in the brain tissue around the hematoma increased at 6 h and peaked at 48 h in the model group, while those in the aprotinin group decreased significantly at each time point, especially 48 and 72 h in the model group and the aprotinin group had no significant difference in neurological deficit score at 61 and 2 hours, and there were significant differences at other time points (P <0.01). Conclusion IL-1β is involved in secondary brain injury after intracerebral hemorrhage. Aprotinin can significantly reduce the expression of IL-1β after intracerebral hemorrhage and thus play a neuroprotective role.