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本实验探讨了ＴＮＦ－α和ＩＦＮ－γ对人胰腺癌ＳＷ－１９９０细胞中ＣＤ４４标准型及变异型蛋白表达的调节作用。分别用２５０Ｕ／ｍｌ的ＴＮＦ－α和ＩＦＮ－γ处理培养中的人胰腺癌细胞株ＳＷ－１９９０，流式细胞和免疫组化方法检测显示，２５０Ｕ／ｍｌ的ＴＮＦ－α和ＩＦＮ－γ均可上调ＳＷ－１９９０细胞中ＣＤ４４变异型蛋白的表达。提示细胞因子的作用是人胰腺癌细胞ＣＤ４４蛋白表达变化的可能机制之一；ＴＮＦ－α和ＩＦＮ－γ可能通过上调肿瘤细胞ＣＤ４４变异型蛋白的表达，而参与了肿瘤的浸润、转移过程。
This experiment explored the regulatory effects of TNF-α and IFN-γ on the expression of CD44 standard and variant proteins in human pancreatic cancer SW-1990 cells. Human pancreatic cancer cell line SW-1990 was treated with 250 U/ml TNF-α and IFN-γ, respectively. Flow cytometry and immunohistochemistry showed that 250 U/ml TNF-α and IFN-γ were Upregulate the expression of CD44 variant protein in SW-1990 cells. It suggests that the role of cytokines is one of the possible mechanisms of human pancreatic cancer cell CD44 protein expression; TNF-α and IFN-γ may be involved in the process of tumor invasion and metastasis by up-regulating the expression of CD44 variant protein in tumor cells.