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目的了解90份婴幼儿腹泻病人轮状病毒分子生物学方法检测阴性的粪便标本中诺瓦克样病毒感染状况。方法应用酶联免疫吸附实验方法(ELISA)进行诺瓦克样病毒抗原检测。结果90份粪便标本中诺瓦克样病毒I型(G1)和Ⅱ型(G2)阳性分别检出6例和19例,阳性率分别为6.67%和21.11%,两者差异有显著性。结论诺瓦克样病毒感染可能是武汉地区婴幼儿腹泻的重要病因之一,其中基因Ⅱ型为主要流行株。
Objective To understand the status of Norwalk-like virus infection in 90 negative stool specimens by molecular biology of rotavirus in 90 infants and children with diarrhea. Methods Norwalk-like virus antigens were detected by enzyme linked immunosorbent assay (ELISA). Results The positive rate of Novak’s type I (G1) and type Ⅱ (G2) in 90 stool specimens were detected in 6 and 19 cases respectively, the positive rates were 6.67% and 21.11%, respectively. There was significant difference between them. Conclusion Norwalk-like virus infection may be an important cause of diarrhea in infants and young children in Wuhan. Among them, genotype Ⅱ is the major epidemic strain.