论文部分内容阅读
目的:通过探讨红色毛癣菌体外分泌角蛋白酶的活性与诱导底物及其基因型的关系,从而了解红色毛癣菌的侵袭力与感染部位及其基因型的关系。方法:分别以指甲、趾甲、皮屑和头发为底物,对不同基因型红色毛癣菌T1a和T1b进行体外培养,利用keratin-azure和酶标仪检测其产生的角蛋白酶活性。结果:不同底物诱导红色毛癣菌T1a和T1b产生角蛋白酶活性的高低比较,指(趾)甲高于皮屑(P<0.05),皮屑高于头发(P<0.05),指甲和趾甲之间差异无统计学意义(P>0.05)。同一底物诱导菌株T1a和T1b产生的角蛋白酶活性间差异无统计学意义(P>0.05)。结论:不同底物诱导的角蛋白酶活性不同;红色毛癣菌的侵袭力可能与其感染部位所含的诱导底物有关,而与基因型无关。
OBJECTIVE: To investigate the relationship between invasiveness of Trichophyton rubrum and the induction substrate and its genotypes in order to understand the relationship between invasiveness of Trichophyton rubrum and infection site and genotype. Methods: The different genotypes of Trichophyton rubrum T1a and T1b were cultured in vitro using nails, toenails, dander and hair as substrate. The keratinase activity was detected by keratin-azure and microplate reader. Results: The results showed that keratinase activity was higher in T1a and T1b of T. rubrum than in different substrates, and the ratio of keratinase activity to DNA was higher than that of dander (P <0.05) and dander was higher than that of hair (P <0.05) The difference was not statistically significant (P> 0.05). The same substrate induced strain T1a and T1b produced keratinase activity was no significant difference (P> 0.05). CONCLUSION: The keratinase activity induced by different substrates is different. The invasiveness of Trichophyton rubrum may be related to the induction substrate contained in the infected site, but not to the genotype.