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研究了Emerson试剂与硫酸沙丁胺醇相互作用所引起吸收光谱的变化。实验发现,在碱性介质中,铁氰化钾氧化硫酸沙丁胺醇的生成物可与4-氨基安替比林发生反应,生成酒红色4-氨基安替比林衍生物,最大吸收波长506 nm,硫酸沙丁胺醇的浓度在0.40~32.0μg/mL范围内与体系的吸光度呈良好的线性关系,其线性回归方程为A=0.014 68+0.046 59c(c,μg/mL;r=0.999 4),方法的检出限为0.020μg/mL,对浓度为8.0μg/mL的硫酸沙丁胺醇平行11次测定,其相对标准偏差为1.3%。据此建立了一种测定硫酸沙丁胺醇的新方法,并应用于硫酸沙丁胺醇片剂中其含量的测定,回收率100.9%~101.4%。
The change of absorption spectrum caused by the interaction between Emerson’s reagent and salbutamol sulfate was studied. The results showed that in alkaline medium, the product of salbutamol sulfate oxidation can react with 4-aminoantipyrine to generate wine red 4-aminoantipyrine derivatives with the maximum absorption wavelength of 506 nm, The concentration of albuterol sulfate in the range of 0.40 ~ 32.0μg / mL showed a good linear relationship with the absorbance of the system. The linear regression equation was A = 0.01468 + 0.046 59c (c, μg / mL; r = 0.999 4) The detection limit was 0.020μg / mL, and the relative standard deviation was 1.3% for 11 parallel determinations of salbutamol sulfate at a concentration of 8.0μg / mL. A new method was established for the determination of salbutamol sulphate. The method was applied to the determination of salbutamol sulphate tablets with the recoveries of 100.9% -101.4%.