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作者用提纯的霍乱毒素免疫BALB/C鼠的脾细胞与骨髓瘤细胞株(SP_2/O)融合,使形成杂交瘤。用放射免疫分析(RIA)筛选了融合后产生的杂交瘤。筛选后通过间接固相放射免疫测定方法对杂交瘤细胞系产生的抗霍乱毒素单克隆抗体的特异性,血清学特性进行鉴定和分析。作者用3只鼠的脾细胞共接种300孔。均见杂交瘤生长,用间接固相RIA方法检测抗霍乱毒素特异性抗体。至少有181孔呈
The authors used purified cholera toxin immunized BALB / c mouse spleen cells and myeloma cell line (SP_2 / O) fusion, the formation of hybridomas. The hybridomas generated after the fusion were screened by radioimmunoassay (RIA). After screening, the specificity and serological characteristics of anti-cholera toxin monoclonal antibodies produced by hybridoma cell lines were identified and analyzed by indirect solid-phase radioimmunoassay. The authors used a total of 300 mouse spleen cells inoculation. Hybridomas were all seen to grow, and anti-cholera toxin-specific antibodies were detected by indirect solid phase RIA. At least 181 holes were present