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在前期通过转录组研究发现1个NBS-LRR类抗病基因(Solyc05g009760.1)在抗TYLCV番茄材料‘CLN2777A’中上调表达,推测可能参与抵抗TYLCV侵染的防卫反应的基础上,以‘CLN2777A’番茄为材料,通过RT-PCR克隆该基因全长cDNA序列,命名为ClNLR。荧光定量PCR发现ClNLR在番茄‘CLN2777A’的根和叶片中高水平表达。ClNLR在本氏烟叶片上的瞬时表达诱导了过敏性反应。病毒诱导的基因沉默(VIGS)抑制ClNLR基因在抗病番茄‘CLN2777A’中表达后,接种TYLCV,检测叶片带毒量,发现VIGS处理的番茄植株体内的TYLCV积累量与其ClNLR基因表达水平成反比。这些研究结果表明ClNLR可能为抗番茄黄化曲叶病的相关基因。
In the previous study, one NBS-LRR resistance gene (Solyc05g009760.1) was up-regulated in trans-TYLCV tomato material ’CLN2777A’ and presumed that it might be involved in the defensive response against TYLCV infection. Based on ’CLN2777A ’Tomato as material, the full-length cDNA sequence of this gene was cloned by RT-PCR and named ClNLR. Fluorescent quantitative PCR revealed that ClNLR was highly expressed in roots and leaves of tomato ’CLN2777A’. Transient expression of ClNLR on N. benthamiana leaves induces anaphylaxis. Virus-induced gene silencing (VIGS) inhibited the expression of ClNLR gene in disease-resistant tomato ’CLN2777A’, and then inoculated with TYLCV to detect leaf virulence. The accumulation of TYLCV in VIGS-treated tomato plants was inversely proportional to the level of ClNLR gene expression. These findings indicate that ClNLR may be a related gene against Tomato yellow leaf curl disease.