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本试验研究了六种培养基系统对马铃薯东农３０３，Ｆａｖｏｒｉｔａ和极早三个品种来源的不同外植体的愈伤组织诱导和植株再生的影响，筛选出了适合植株再生的最佳外植体、最佳外植体大小及最佳培养基系统。适合植株再生的最佳外植体为叶片；最佳外植体大小为叶片０．５ｃｍ２、茎０．５ｃｍ、块茎０．２５ｃｍ２×０．３ｃｍ，适合于茎、块茎及叶的有效培养基系统分别为：①ＭＳ＋３ｍｇ·Ｌ-１ＢＡ＋０．０１ｍｇ·Ｌ-１ＮＡＡ（ｐｈ５．６）４周ＭＳ＋０．３ｍｇ·Ｌ-１ＧＡ３（ｏＨ５．６）；②ＭＳ＋１．７５ｍｇ·Ｌ-１ＺＴ＋０．８７ｍｇ·Ｌ-１ＩＡＡ（ｐＨ５．８）；③ＭＳ＋２．２５ｍｇ·Ｌ-１ＢＡ＋０．１８６ｍｇ·Ｌ-１ＮＡＡ（ｐＨ５．８）２周ＭＳ＋５ｍｇ·Ｌ-１ＧＡ３＋２．２５ｍｇ·Ｌ-１ＢＡ（ｐｈ５．８）。
In this study, we investigated the effects of six media systems on the callus induction and plant regeneration of different explants derived from potato Dongnong303, Favorita and very early three cultivars, and screened out the best explants for plant regeneration Body, best explant size and best culture system. The best explants suitable for plant regeneration were leaves. The best explants were 0.5 cm 2 in leaf size, 0.5 cm in stem and 0.25 cm × 0.3 cm in tuber size, which was suitable for effective culture system of stem, tuber and leaf Respectively: MS + 3mg · L-1BA + 0.01mg · L-1NAA (ph5.6) 4 weeks MS + 0.3mg · L-1GA3 (oH5.6); ②MS + 1.75mg · L-1ZT + 0.87mg · L-1IAA (pH5) 8); MS + 2.25 mg.L-1BA + 0.186 mg.L-1 NAA (pH 5.8) for 2 weeks MS + 5 mg.L-1GA3 + 2.25 mg.L-1BA (ph5.8).