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用基因芯片技术研究高(H)、低(L)转移卵巢癌细胞株(HO-8910PM和HO-8910)和正常卵巢上皮(C)基因表达谱差异,筛选与卵巢癌转移相关的基因,并利用生物信息学方法对检测结果进行差异基因在染色体定位和功能分析。结果:高、低转移卵巢癌细胞株比较表达差异2倍以上共有409个基因,其中表达上调(信号比的对数值[SLR]≥1)有271个,表达下调(SLR≤-1)有138个。从表达差异的基因在染色体定位分析,发现除1个基因未知其定位外,其余所有差异表达基因散在分布于各条染色体上,但以1号染色体最多有43个(占10．7％)。其次是6号染色体有39个(占9．6％),第三是2号染色体有29个(占7．1％)。第四是17号染色体有28个(占6．9％)。第五是3号染色体有25个(占6．2％)。第6是5号和11号染色体各有24个(各占5．9％)。而差异表达的基因发生在染色体短臂(q)的有264个(占64．7％),在13, 14,15,21和22号仅发现在q都有异常表达。从表达差异基因的分子功能分类看,属于酶和酶调控子基因为最多(104个,占25．4％),其次是信号传导基因(43个,占10．5％)。第3类是核酸结合基因(42个,占10．3％)。第4类是蛋白结合基因(34个,占8．3％)。以上4大类共占基因总数54．5％。还有功能未知的基因有76个,占18．6％。高、低转移卵巢癌细胞株差异表达基因散在分布在各条染色体上,但以1、6、2、17、3、5和11号染色体差异表达基因居多。肿瘤的转移是多基因共同作用的结果。4大类(酶和酶调控子、信号传导、核酸结合和蛋白结合)相关基因异常是我们今后研究卵巢癌转移的重要基因。
Gene chip technology was used to study the difference of gene expression profiles in high (H), low (L) metastatic ovarian cancer cell lines (HO-8910PM and HO-8910) and normal ovarian epithelium (C) The bioinformatics method was used to analyze the difference of the genes in chromosome location and function. RESULTS: A total of 409 genes were expressed more than 2-fold in high and low metastatic ovarian cancer cell lines, with 271 up-regulated (signal to log ratio [SLR] ≥1) and 138 down-regulated A All of the differentially expressed genes were scattered on each chromosome except one locus, except for one locus. However, no more than 43 loci (10.7%) were found on chromosome 1. Followed by 39 chromosomes (9.6%) on chromosome 6 and 29 (7.1%) on chromosome 2. The fourth is 28 on chromosome 17 (6.9%). The fifth is 25 on chromosome 3 (6.2%). Sixth, chromosomes 5 and 11 each have 24 (5.9% each). The differentially expressed genes were found in 264 (64.7%) of the short arm (q) of the chromosome, and only abnormally expressed in q at 13, 14, 15, 21 and 22. According to the molecular functional classification of differentially expressed genes, the genes belonging to enzymes and enzyme regulators are the most (104, accounting for 25.4%), followed by the signal transduction genes (43, accounting for 10.5%). The third category is the nucleic acid-binding genes (42, accounting for 10.3%). The fourth category is the protein binding gene (34, accounting for 8.3%). The above four categories accounted for 54.5% of the total number of genes. There are 76 unknown genes, accounting for 18.6%. The differentially expressed genes of high and low metastatic ovarian cancer cell lines were scattered on each chromosome, but most of the differentially expressed genes on chromosomes 1, 6, 2, 2, 3, 5 and 11 were found. Tumor metastasis is the result of multi-gene interactions. 4 categories (enzymes and enzyme regulators, signal transduction, nucleic acid binding and protein binding) related gene abnormalities is our future study of ovarian cancer metastasis of important genes.